![]() ![]() Visit the I NTEGRA Biosciences website to learn more, or use the Request Information button to make direct contact. Advanced sequencing protocol for difficult-to-sequence samples. In my opinion, INTEGRA handheld electronic pipettes are impressive as they solved the complications in our lab, and I truly believe they outperform 1, 8, and 12 channel pipettes – I love it!” GMP sequencing) Next generation sequencing (de novo & re-sequencing at the genome. Nextera XT Sample Preparation Kit (Illumina, DM basis (Krger et al., 2017). DNA/RNA ANALYSES AND SEQUENCING DNA Sanger sequencing of all types (incl. Being able to keep a close eye on the speed of liquid aspiration and dispensing helps to avoid any loss, and reduces the likelihood of poor sample recovery, ensuring that quality standards are matched and expectations are met. Illumina MiSeq paired-ends sequencing technology (Microsynth AG, Balgach. The VIAFLO 96, on the other hand, is used in cfDNA isolation to pipette samples with different viscosities over a wide volume range, which would otherwise be difficult to program on automated systems. All these applications require precise, simultaneous transfer of multiple liquid samples the MINI 96 has allowed us to do exactly this and, best of all, we get more hands-off time. If you do so, please provide us with the gel picture or trace file as well. However, we do recommend that you also check your DNA on a gel or on the Bioanalyzer. “We use the MINI 96 for digital PCR and qPCR preparation, as well as in end-point PCR reactions and library preparation for NGS. Microsynth performs on each received sample a complete quality control prior to further sequencing steps. We use the MINI 96 and VIAFLO 96 in every step of sample preparation to achieve this high throughput.” primer (27F) and sent to Microsynth Company for sequencing (Switzerland). Scientists at Microsynth – a leading European company in nucleic acid synthesis, analysis, and sequencing – are taking advantage of the remarkable liquid handling capabilities of INTEGRA’s MINI 96 and VIAFLO 96 handheld electronic pipettes in workflows ranging from nucleic acid isolation to sequencing and different types of PCR.ĭr Sergey Yakushev, Head of Genetic Analysis at Microsynth, explained: “We receive as many as 20,000 samples per year from our customers, so we do a lot of preparation and analysis that requires us to perform multiple liquid handling steps. United States) and following the manufacturer's protocol, except that sterile. E.coli (Ecoli NightSeq®) add 1 colony (diameter 1 mm) OR. ![]()
0 Comments
Leave a Reply. |